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Journal: Molecular Therapy Oncology
Article Title: CBX6 and CA9 as predictive indicators and therapeutic targets in GBM
doi: 10.1016/j.omton.2026.201159
Figure Lengend Snippet: Hypoxia impacts gene expression and signaling pathways in GBM cells (A) Hypoxia-responsive pathways were identified using Partek Flow software under double filter MI50 and fold change >+1.5 criteria. (B) Expression of candidate genes in PBT030 cells following 48-h hypoxia exposure compared to cells under normoxic conditions. (C) Expression of candidate genes in U-87 MG cells after 48 h of hypoxia compared to cells maintained under normoxic conditions. Representative bar graphs are derived from at least two independent experiments, with 28S used as the reference gene for all experiments.
Article Snippet: U-251 MG and
Techniques: Gene Expression, Protein-Protein interactions, Software, Expressing, Derivative Assay
Journal: Science Advances
Article Title: An engineered insulin analog with dual insulin and IGF-1 receptor agonism and distinct signaling
doi: 10.1126/sciadv.aeb7558
Figure Lengend Snippet: Cells expressing IGF-1R ( A and E ), SH-SY5Y ( B and F ), U87MG ( C and G ), and rat primary neuronal culture ( D and H ) were stimulated with 10 nM ligands for 20 min. Phosphorylation of Akt (A to D) and Erk1/2 (E to H) was assessed by Western blot. Data were normalized to GAPDH loading control and are shown relative to IGF-1–induced signal. Identical GAPDH loading controls are shown for the figure pairs (A) and (E), (B) and (F), and (D) and (H) because the samples in each pair were run on the same gel. Representative blots are shown and all blots are shown in the Supplementary Materials. Color coding as in . Asterisks indicate significance versus IGF-1 [(A), (B), and (E)] or versus control [(C), (D), (F), (G), and (H)]. (* P < 0.05; ** P < 0.01; *** P < 0.001; # P < 0.0001; ns, not significant according to ANOVA). X indicates that this sample was not included in the study.
Article Snippet: The
Techniques: Expressing, Phospho-proteomics, Western Blot, Control